Regulatory

Part:BBa_K080001:Design

Designed by: Jennifer Lei   Group: iGEM08_Bay_Area_RSI   (2008-10-20)

A20/alpha cardiac actin miniPro-BMP4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
    Illegal SpeI site found at 766
    Illegal PstI site found at 813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1385
    Illegal SpeI site found at 766
    Illegal PstI site found at 813
    Illegal NotI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
    Illegal SpeI site found at 766
    Illegal PstI site found at 813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
    Illegal SpeI site found at 766
    Illegal PstI site found at 813
    Illegal NgoMIV site found at 109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 239
    Illegal SapI.rc site found at 443


Design Notes

A20/alpha cardiac actin minpromoter – BMP4  Late inducer of Cardiac Muscle Differentiation Cla1(6bp)-Xba1(6bp)-A20/a-CA minimal Pro (116bp) – pac1 (8bp) – mlu1 (6bp) – kozak (6bp) -BMP4 (1223bp) - Nhe1 (6bp) – sal1 (6bp) = 1383bp

Source

The A20/alpha-CA minimal promoter was created synthetically via oligonucleotide assembly.

BMP4 Mus musculus full length cDNA clone from openbiosystems, clone ID# 4219098


References